Frangakis


      Group

Job Positions

Ph.D. Position in Biochemistry Cryo-Electron Microscopy / Tomography

The cryo-electron microscopy group at the Buchmann Institute for Molecular Life Sciences is seeking an enthusiastic, highly motivated Ph.D. student with a background in biochemistry/molecular biology to occupy a PhD position.

The scientific goal of the project is to gain knowledge into the transcription apparatus using cryo-electron microscopy. To achieve this, we use advanced biochemical methods and a brand-new cutting-edge visualization technology called cryo-electron tomography. This allows us to look directly into the nucleus and see the individual enzymes and the DNA giving unlimited possibilities for exploration. In this project, we would like to study the RNA polymerase at all operational states in the unperturbed cellular context in order to gain a deep understanding of its function and regulation. Further, individual enzymes can be studied by single particle cryo-electron microscopy at atomic resolution. This project is embedded within the SFB 902 (Molecular Principles of RNA-based Regulation).  

Do you have a degree in biochemistry and/or molecular biology, excellent grades and a strong interest in structural biology and electron microscopy? Previous experience working with large macromolecules such as the ribosome or RNA polymerase is advantageous. Technically, experience with cell culture, cloning, fluorescence tagging, as well as some experience in protein purification would be helpful. 

Interested candidates should send a short application letter outlining their research interests, a detailed CV to Professor Achilleas Frangakis (email: achilleas (dot) frangakis (at) biophysik.org)

Ph.D. Position or Scientist Position Computer Science

The electron microscopy group at the Buchmann Institute for Molecular Life Sciences is seeking an enthusiastic, highly motivated scientist (Ph.D. or Postdoctoral level) with a background in computer science (Informatik).

The scientific goal of the project is to develop algorithms that reconstruct three-dimensional objects out of their 2D projections. Experimental images from brand-new cutting-edge technologies are available (hardware and software). Typically, these unique imaging capabilities are used to visualize the interior of the cells that allows for understanding the origin and cause of cancer and other disease.  

Previous experience with GPU programming knowledge (CUDA, OpenCL) and a solid knowledge of Fourier analysis is a prerequisite. Knowledge in C, C++ and MATLAB programming experience is a big advantage. The tasks will be used for developing code for image processing and/or computed tomography for medical applications. Thus, a general interest in medical imaging is advantageous. 

Do you have a degree in mathematics or computer science and want to get into the medical imaging field. Do you have excellent grades and a strong interest in programming and algorithmic development? Would you like to develop software and tools for 3D visualization? We are a small team of passionate individuals. Interested candidates should send a short application letter outlining their research interests, a detailed CV to Prof. Dr. Achilleas Frangakis (email: achilleas (dot) frangakis (at) biophysik.org)

%MCEPASTEBIN%

Zwei Masterarbeiten 

In der Arbeitsgruppe Frangakis am BMLS (Campus Riedberg) haben wir aktuell zwei Masterarbeiten zu vergeben. Es besteht die Möglichkeit während der Masterarbeit zusätzlich als wissenschaftliche Hilfskraft bei uns beschäftigt zu werden, um eng verwandte Fragestellungen zu bearbeiten und finanziell unterstützt zu werden. Wir sind eine interdisziplinäre Gruppe von Biologen, Informatikern und Physikern, die (Kryo-) Transmissions-Elektronenmikroskopie benutzt, um Zellen und kleine Organismen mit molekularer Auflösung abzubilden. Unsere Tomogramme sind komplexe dreidimensionale Bilder aus denen die Informationen computergestützt gewonnen werden. Aktuell beschäftigen wir uns insbesondere mit der hochauflösenden Darstellung von Zell-Zell-Kontakten (primäre Gewebekulturzellen und Leberproben). Ebenfalls betreiben wir klassische, biologische Raumtemperaturelektronenmikroskopie, wobei der Erhalt der Ultrastruktur durch Hochdruckgefrieren unterstützt wird. Dieses Verfahren benutzen wir insbesondere zur Abbildung größerer Volumina wie ganzer Modellorganismen (C. elegans oder Drosophilaembryos) oder Gewebe (aktuell: Darstellung der Blut-Hirn Schranke in Mäusehirnen, Analyse der Filtration durch die Schlitzmembran der Niere) mittels Rasterelektronenmikroskopie (FIB-SEM). Vorerfahrungen in der Elektronenmikroskopie sind nicht notwendig, ein generelles Interesse an bildgebenden Verfahren ist jedoch essentiell. Bei Interesse und für weitere Informationen senden Sie uns bitte eine Email an: This email address is being protected from spambots. You need JavaScript enabled to view it.