Our goal is to reveal novel macromolecular interactions, up to the level of whole cells, by means of cryo-electron tomography and advanced pattern recognition algorithms. Cryo-electron tomography is the only technique that can obtain molecular resolution images of intact cells in a quasi-native environment.
FIB-SEM and Electron Tomography
In addition, we are using classical biological room temperature electron micoscopy. Ultrastructural preservation is assured in these preparations by prior high pressure freezing. We use this method to image larger volumina, as e.g. tissues and whole model organisms (currently C. elegans and Drosophila embryos) by focussed ion beam scanning electron microscopy and (after ultramicrotomy) by room temperature tomography.
Section of human tissue at molecular resolution
Ph.D. and Post Doc Positions
Diploma, Master and Bachelor Theses
Performance evaluation of image processing algorithms on the GPU
Castano-Diez, D., Moser, D., Schoenegger, A., Pruggnaller, S., Frangakis, A.S.
J Struct Biol. 2008 Oct; 164(1):153-60. Epub 2008 Jul 24.
A visualization and segmentation toolbox for electron microscopy
Pruggnaller, S., Mayr, M., Frangakis, A.S.
J Struct Biol. 2008 Oct; 164(1):161-5. Epub 2008 May 17.
The molecular architecture of cadherins in native epidermal desmosomes.
Al-Amoudi, A., Diez, D.C., Betts, M.J. & Frangakis, A.S.
Nature. 2007 Dec 6;450(7171):832-7.
Fiducial-less alignment of cryo-sections.
Castano-Diez, D., Al-Amoudi, A., Glynn, A.M., Seybert, A. & Frangakis, A.S.
J Struct Biol. 2007 Sep;159(3):413-23. Epub 2007 Jun 9.
Cryo-electron tomography reveals the cytoskeletal structure of Spiroplasma melliferum.
Kurner, J., Frangakis, A.S. & Baumeister, W.
Science 2005 Jan 21;307(5708):436-8.