Frangakis


      Group

Cryo-Electron Microscopy

 
Our goal is to reveal novel macromolecular interactions, up to the level of whole cells, by means of cryo-electron tomography and advanced pattern recognition algorithms. Cryo-electron tomography is the only technique that can obtain molecular resolution images of intact cells in a quasi-native state.

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FIB-SEM and Electron Tomography

 
In addition, we are using classical biological room temperature electron micoscopy. Ultrastructural preservation is assured in these preparations by prior high pressure freezing. We use this method to image larger volumina, as e.g. tissues and whole model organisms (currently C. elegans and Drosophila embryos) by focussed ion beam scanning electron microscopy and (after ultramicrotomy) by room temperature tomography.

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Teaching

We offer special lectures and courses in Electron Microscopy and Image Processing for physic, biophysic, biochemistry, chemistry and biology students. Our aim is to teach you the main principles of both methods and give you an understanding for their impact in modern Life Sciences.

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Contact

Frangakis Group Cluster of Excellence Macromolecular Complexes
Johann Wolfgang Goethe University Frankfurt Institute of Biophysics Max-von-Laue-Str. 15
D-60438 Frankfurt am Main Germany.

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FCEM and Electron Microscopy access for the public

The primary aim of the FCEM is to promote the application of electron microscopy in all research disciplines of the Goethe University. Past and current users of the Goethe University have been from the departments of Molecular Biology, Cellular Biology, Neuroscience, Medicine, Biochemistry, Pharmacy, and Physics. FCEM has been also utilized by external institutions such as the University of Zurich, Max-Planck-Institute for Biophysical Chemistry and others, as well as from non-academic users.

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Recent publications

Three-dimensional CTF correction improves the resolution of electron tomograms. Kunz M, Frangakis AS. J Struct Biol. 2016 Jun 23. pii: S1047-8477(16)30122-8. doi: 10.1016/j.jsb.2016.06.016. PubMed PMID: 27343995

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